The protest took place on Jules Rutherford’s first day as Labour’s new head of membership on Wednesday.
Politics Home revealed how Ms Rutherford had retweeted a post which claimed anti-semitism allegations were "smears against the party leader".
Shortly after Labour were contacted about it, Ms Rutherford's Twitter account was deleted.
It is understood that her appointment caused anger among staff in Labour's Newcastle office, where the party's membership department is based.
A Labour source said: "On her first day, she went around desks meeting colleagues - only they all walked out of the office because they didn’t want to meet her."
One member of staff did remain in the office, but only to challenge Ms Rutherford on her views on anti-semitism and to ask why she had retweeted the "smear" video.
A Labour spokesperson refused to comment.
It seems Labour like the Socialist Party (see post below) treat their employees with disdain. At least the Labour staff belong to a trade union. They obviously need one. Their Branch passed the following motion as reported by Politics Home previously:
"Whistleblowers do so at enormous personal risk and should be commended and supported, never attacked – particularly not by the Labour Party
"All employees or former employees, regardless of the organisation, should be free to act as whistleblowers without obscene threats of legal action."
It "condemned the Labour Party’s official response to the Panorama programme and the attacks on former staff who we have found to be committed, principled and honest".
The motion went on to "demand that the Labour Party apologises to former colleagues for the attacks launched on them by both the Labour Party officially and approved “outriders” over the Panorama programme which highlighted the problem of anti-semitism within the Labour Party".
Meanwhile the Jewish Leadership Council writes:
Labour Against Anti-Semitism responded with the following statement: